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template for pcr  (TaKaRa)


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    TaKaRa template for pcr
    Template For Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 968 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/template+for+pcr/CloneAmp+HiFi+PCR+Premix/bio_rxiv__2025__10__31__685944-230-6-11
    Average 96 stars, based on 968 article reviews
    template for pcr - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    BAC Assay:

    Article Title: hCLCA2 Is a p53-Inducible Inhibitor of Breast Cancer Cell Proliferation
    Article Snippet: .. A BAC clone containing the hCLCA2 promoter region (#RK11-268K17; Oakland Research Institute) was used as template for PCR (Prime-Star; Takara). .. Error-free pGEM-T clones were inserted into pGL4 luciferase vector and cotransfected with Renilla control phRL-TK into 293T cells at a ratio of 25:1 using Lipofectamine2000 (Invitrogen).

    Article Title: hCLCA2 is a p53-inducible inhibitor of breast cancer cell proliferation
    Article Snippet: Recombinant adenovirus was then generated according to established protocols, and viral supernatants were titrated on HEK293 cells and on each breast cell line. p53-AdEasy was obtained from Vector Labs. Adherent MCF10A, CA1D, and BT549 cells were typically infected overnight at an MOI of 20. .. A BAC clone containing the hCLCA2 promoter region (#RK11-268K17, Oakland Research Institute) was used as template for PCR (Prime-Star, Takara). .. Error-free pGEM-T clones were inserted into pGL4 luciferase vector and co-transfected with Renilla control phRL-TK into 293T cells at a ratio of 25:1 using Lipofectamine2000 (Invitrogen).

    Polymerase Chain Reaction:

    Article Title: hCLCA2 Is a p53-Inducible Inhibitor of Breast Cancer Cell Proliferation
    Article Snippet: .. A BAC clone containing the hCLCA2 promoter region (#RK11-268K17; Oakland Research Institute) was used as template for PCR (Prime-Star; Takara). .. Error-free pGEM-T clones were inserted into pGL4 luciferase vector and cotransfected with Renilla control phRL-TK into 293T cells at a ratio of 25:1 using Lipofectamine2000 (Invitrogen).

    Article Title: Lipid bilayer thinning near a ubiquitin ligase selects ER membrane proteins for degradation
    Article Snippet: The collected cells were grown overnight, spun, and plasmids were extracted using the yeast plasmid extraction kit (ZymoResearch #D2004). .. Extracted plasmids were used as a template for PCR (CloneAmp polymerase, Takara #639298) to amplify a fragment containing the TM sequence using primers flanked with Illumina adaptors. .. PCR reactions were purified using the QIAquick PCR purification kit (Qiagen #28104), and confirmed to yield a single (∼350bp) fragment by agarose gel electrophoresis.

    Article Title: hCLCA2 is a p53-inducible inhibitor of breast cancer cell proliferation
    Article Snippet: Recombinant adenovirus was then generated according to established protocols, and viral supernatants were titrated on HEK293 cells and on each breast cell line. p53-AdEasy was obtained from Vector Labs. Adherent MCF10A, CA1D, and BT549 cells were typically infected overnight at an MOI of 20. .. A BAC clone containing the hCLCA2 promoter region (#RK11-268K17, Oakland Research Institute) was used as template for PCR (Prime-Star, Takara). .. Error-free pGEM-T clones were inserted into pGL4 luciferase vector and co-transfected with Renilla control phRL-TK into 293T cells at a ratio of 25:1 using Lipofectamine2000 (Invitrogen).

    Sequencing:

    Article Title: Lipid bilayer thinning near a ubiquitin ligase selects ER membrane proteins for degradation
    Article Snippet: The collected cells were grown overnight, spun, and plasmids were extracted using the yeast plasmid extraction kit (ZymoResearch #D2004). .. Extracted plasmids were used as a template for PCR (CloneAmp polymerase, Takara #639298) to amplify a fragment containing the TM sequence using primers flanked with Illumina adaptors. .. PCR reactions were purified using the QIAquick PCR purification kit (Qiagen #28104), and confirmed to yield a single (∼350bp) fragment by agarose gel electrophoresis.



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    A , Quantitative RT‐PCR analysis of <t>TACR1</t> , TACR2 , and TACR3 mRNAs in 42 independent APAs. Each dot indicates 1 adenoma. Data are presented as mean±SEM. P =0.012; P <0.0001. B , Comparison of the expression levels of the TACR1 gene between different APA subgroups. classical APAs; nonclassical APAs (left panel) P =0.045; NKD and KCNJ5 (middle panel); CYP11B2‐positive nodules in nonclassical APAs (<2 nodules vs ≥2 nodules, right panel). C through F , Distribution of NK1R immunostaining in APA and adjacent adrenal tissue representative of n=56 independent APAs. C, E, F, NK1R staining in a classical APA and its adjacent adrenal cortex. C , Overall NK1R staining pattern in an APA and the adjacent adrenal cortex. D , Closer view of the NK1R staining in a different classical APA, highlighting areas of staining present in the zona glomerulosa or organized in micronodules within the adrenal tissue adjacent to the adenoma (arrows). E , Close‐up view of NK1R‐positive staining in the ZG and nerve ganglia in the adrenal cortex adjacent to the adenoma). F , High magnification view illustrating the distribution of NK1R staining in a group of adenoma cells. Arrow and arrowhead show membrane and cytoplasmic NK1R staining, respectively. APA indicates aldosterone‐producing adenoma; C, classical; Ca, capsule; G, nerve ganglia; KCNJ5 , KCNJ5 mutation detected; NC, nonclassical; NKD, no KCNJ5 mutation detected; NK1R, neurokinin type 1 receptor; RT‐PCR, reverse transcription polymerase chain reaction; and ZG, zona glomerulosa.
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    A , Quantitative RT‐PCR analysis of <t>TACR1</t> , TACR2 , and TACR3 mRNAs in 42 independent APAs. Each dot indicates 1 adenoma. Data are presented as mean±SEM. P =0.012; P <0.0001. B , Comparison of the expression levels of the TACR1 gene between different APA subgroups. classical APAs; nonclassical APAs (left panel) P =0.045; NKD and KCNJ5 (middle panel); CYP11B2‐positive nodules in nonclassical APAs (<2 nodules vs ≥2 nodules, right panel). C through F , Distribution of NK1R immunostaining in APA and adjacent adrenal tissue representative of n=56 independent APAs. C, E, F, NK1R staining in a classical APA and its adjacent adrenal cortex. C , Overall NK1R staining pattern in an APA and the adjacent adrenal cortex. D , Closer view of the NK1R staining in a different classical APA, highlighting areas of staining present in the zona glomerulosa or organized in micronodules within the adrenal tissue adjacent to the adenoma (arrows). E , Close‐up view of NK1R‐positive staining in the ZG and nerve ganglia in the adrenal cortex adjacent to the adenoma). F , High magnification view illustrating the distribution of NK1R staining in a group of adenoma cells. Arrow and arrowhead show membrane and cytoplasmic NK1R staining, respectively. APA indicates aldosterone‐producing adenoma; C, classical; Ca, capsule; G, nerve ganglia; KCNJ5 , KCNJ5 mutation detected; NC, nonclassical; NKD, no KCNJ5 mutation detected; NK1R, neurokinin type 1 receptor; RT‐PCR, reverse transcription polymerase chain reaction; and ZG, zona glomerulosa.
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    A , Quantitative RT‐PCR analysis of <t>TACR1</t> , TACR2 , and TACR3 mRNAs in 42 independent APAs. Each dot indicates 1 adenoma. Data are presented as mean±SEM. P =0.012; P <0.0001. B , Comparison of the expression levels of the TACR1 gene between different APA subgroups. classical APAs; nonclassical APAs (left panel) P =0.045; NKD and KCNJ5 (middle panel); CYP11B2‐positive nodules in nonclassical APAs (<2 nodules vs ≥2 nodules, right panel). C through F , Distribution of NK1R immunostaining in APA and adjacent adrenal tissue representative of n=56 independent APAs. C, E, F, NK1R staining in a classical APA and its adjacent adrenal cortex. C , Overall NK1R staining pattern in an APA and the adjacent adrenal cortex. D , Closer view of the NK1R staining in a different classical APA, highlighting areas of staining present in the zona glomerulosa or organized in micronodules within the adrenal tissue adjacent to the adenoma (arrows). E , Close‐up view of NK1R‐positive staining in the ZG and nerve ganglia in the adrenal cortex adjacent to the adenoma). F , High magnification view illustrating the distribution of NK1R staining in a group of adenoma cells. Arrow and arrowhead show membrane and cytoplasmic NK1R staining, respectively. APA indicates aldosterone‐producing adenoma; C, classical; Ca, capsule; G, nerve ganglia; KCNJ5 , KCNJ5 mutation detected; NC, nonclassical; NKD, no KCNJ5 mutation detected; NK1R, neurokinin type 1 receptor; RT‐PCR, reverse transcription polymerase chain reaction; and ZG, zona glomerulosa.
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    Pcr Template, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A , Quantitative RT‐PCR analysis of <t>TACR1</t> , TACR2 , and TACR3 mRNAs in 42 independent APAs. Each dot indicates 1 adenoma. Data are presented as mean±SEM. P =0.012; P <0.0001. B , Comparison of the expression levels of the TACR1 gene between different APA subgroups. classical APAs; nonclassical APAs (left panel) P =0.045; NKD and KCNJ5 (middle panel); CYP11B2‐positive nodules in nonclassical APAs (<2 nodules vs ≥2 nodules, right panel). C through F , Distribution of NK1R immunostaining in APA and adjacent adrenal tissue representative of n=56 independent APAs. C, E, F, NK1R staining in a classical APA and its adjacent adrenal cortex. C , Overall NK1R staining pattern in an APA and the adjacent adrenal cortex. D , Closer view of the NK1R staining in a different classical APA, highlighting areas of staining present in the zona glomerulosa or organized in micronodules within the adrenal tissue adjacent to the adenoma (arrows). E , Close‐up view of NK1R‐positive staining in the ZG and nerve ganglia in the adrenal cortex adjacent to the adenoma). F , High magnification view illustrating the distribution of NK1R staining in a group of adenoma cells. Arrow and arrowhead show membrane and cytoplasmic NK1R staining, respectively. APA indicates aldosterone‐producing adenoma; C, classical; Ca, capsule; G, nerve ganglia; KCNJ5 , KCNJ5 mutation detected; NC, nonclassical; NKD, no KCNJ5 mutation detected; NK1R, neurokinin type 1 receptor; RT‐PCR, reverse transcription polymerase chain reaction; and ZG, zona glomerulosa.
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    A , Quantitative RT‐PCR analysis of <t>TACR1</t> , TACR2 , and TACR3 mRNAs in 42 independent APAs. Each dot indicates 1 adenoma. Data are presented as mean±SEM. P =0.012; P <0.0001. B , Comparison of the expression levels of the TACR1 gene between different APA subgroups. classical APAs; nonclassical APAs (left panel) P =0.045; NKD and KCNJ5 (middle panel); CYP11B2‐positive nodules in nonclassical APAs (<2 nodules vs ≥2 nodules, right panel). C through F , Distribution of NK1R immunostaining in APA and adjacent adrenal tissue representative of n=56 independent APAs. C, E, F, NK1R staining in a classical APA and its adjacent adrenal cortex. C , Overall NK1R staining pattern in an APA and the adjacent adrenal cortex. D , Closer view of the NK1R staining in a different classical APA, highlighting areas of staining present in the zona glomerulosa or organized in micronodules within the adrenal tissue adjacent to the adenoma (arrows). E , Close‐up view of NK1R‐positive staining in the ZG and nerve ganglia in the adrenal cortex adjacent to the adenoma). F , High magnification view illustrating the distribution of NK1R staining in a group of adenoma cells. Arrow and arrowhead show membrane and cytoplasmic NK1R staining, respectively. APA indicates aldosterone‐producing adenoma; C, classical; Ca, capsule; G, nerve ganglia; KCNJ5 , KCNJ5 mutation detected; NC, nonclassical; NKD, no KCNJ5 mutation detected; NK1R, neurokinin type 1 receptor; RT‐PCR, reverse transcription polymerase chain reaction; and ZG, zona glomerulosa.
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    Image Search Results


    A , Quantitative RT‐PCR analysis of TACR1 , TACR2 , and TACR3 mRNAs in 42 independent APAs. Each dot indicates 1 adenoma. Data are presented as mean±SEM. P =0.012; P <0.0001. B , Comparison of the expression levels of the TACR1 gene between different APA subgroups. classical APAs; nonclassical APAs (left panel) P =0.045; NKD and KCNJ5 (middle panel); CYP11B2‐positive nodules in nonclassical APAs (<2 nodules vs ≥2 nodules, right panel). C through F , Distribution of NK1R immunostaining in APA and adjacent adrenal tissue representative of n=56 independent APAs. C, E, F, NK1R staining in a classical APA and its adjacent adrenal cortex. C , Overall NK1R staining pattern in an APA and the adjacent adrenal cortex. D , Closer view of the NK1R staining in a different classical APA, highlighting areas of staining present in the zona glomerulosa or organized in micronodules within the adrenal tissue adjacent to the adenoma (arrows). E , Close‐up view of NK1R‐positive staining in the ZG and nerve ganglia in the adrenal cortex adjacent to the adenoma). F , High magnification view illustrating the distribution of NK1R staining in a group of adenoma cells. Arrow and arrowhead show membrane and cytoplasmic NK1R staining, respectively. APA indicates aldosterone‐producing adenoma; C, classical; Ca, capsule; G, nerve ganglia; KCNJ5 , KCNJ5 mutation detected; NC, nonclassical; NKD, no KCNJ5 mutation detected; NK1R, neurokinin type 1 receptor; RT‐PCR, reverse transcription polymerase chain reaction; and ZG, zona glomerulosa.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Regulation of Aldosterone Secretion by Substance P and the Neurokinin Type 1 Receptor in Aldosterone‐Producing Adenomas

    doi: 10.1161/JAHA.125.045539

    Figure Lengend Snippet: A , Quantitative RT‐PCR analysis of TACR1 , TACR2 , and TACR3 mRNAs in 42 independent APAs. Each dot indicates 1 adenoma. Data are presented as mean±SEM. P =0.012; P <0.0001. B , Comparison of the expression levels of the TACR1 gene between different APA subgroups. classical APAs; nonclassical APAs (left panel) P =0.045; NKD and KCNJ5 (middle panel); CYP11B2‐positive nodules in nonclassical APAs (<2 nodules vs ≥2 nodules, right panel). C through F , Distribution of NK1R immunostaining in APA and adjacent adrenal tissue representative of n=56 independent APAs. C, E, F, NK1R staining in a classical APA and its adjacent adrenal cortex. C , Overall NK1R staining pattern in an APA and the adjacent adrenal cortex. D , Closer view of the NK1R staining in a different classical APA, highlighting areas of staining present in the zona glomerulosa or organized in micronodules within the adrenal tissue adjacent to the adenoma (arrows). E , Close‐up view of NK1R‐positive staining in the ZG and nerve ganglia in the adrenal cortex adjacent to the adenoma). F , High magnification view illustrating the distribution of NK1R staining in a group of adenoma cells. Arrow and arrowhead show membrane and cytoplasmic NK1R staining, respectively. APA indicates aldosterone‐producing adenoma; C, classical; Ca, capsule; G, nerve ganglia; KCNJ5 , KCNJ5 mutation detected; NC, nonclassical; NKD, no KCNJ5 mutation detected; NK1R, neurokinin type 1 receptor; RT‐PCR, reverse transcription polymerase chain reaction; and ZG, zona glomerulosa.

    Article Snippet: Each sample was analyzed in duplicates, and cDNA quantification was normalized to PPIA (cyclophilin) using the ΔCt method and standard curves generated from polyA mRNAs or TACR1 human quantitative PCR template ( HK210362 , Origene, Rockville, USA).

    Techniques: Quantitative RT-PCR, Comparison, Expressing, Immunostaining, Staining, Membrane, Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Polymerase Chain Reaction